Biochemical Tests
Benedicts test for reducing
sugars
- grind up sample
- add Benedicts solution
- heat
- colour change from blue to red/brown
indicate reducing sugars
- note simple non reducing sugars
(mainly disaccharides) can all be hydrolysed to their reducing
sugar components by heating with dilute acid (e.g. HCl). If you
neutralise after heating you can then perform the Benedicts test
- a positive result indicates
the presence of a simple non-reducing sugar
Iodine (I2) test for starch
- add drops of Iodine to sample
- colour change from brown to
blue black indicates presence of starch
Shultz's test for cellulose
- add Shultz's solution
- purple colour indicates presence
of cellulose
Biuret test for protein
- grind up sample
- add Biuret solution
- lilac colour indicates protein
present
Emulsion test for lipids
- grind up sample
- add ethanol
- decant into water
- cloudy emulsion indicates presence
of lipid